Sliding into third on Edwin Encarnacion's double play ball, Bautista's lead foot could be seen sticking to the ground as he slid. He walked off the field under his own power but was replaced at third base by John McDonald for the top of the fifth.
Bautista had a hit and a walk in three plate appearances before leaving the game.
On the Blue Jays' broadcast, general manager Alex Anthopoulos said Bautista's injury was a "twisted right ankle."
Bautista is arguably the American League MVP so far this season, hitting .334/.468/.702 with 31 home runs and 65 RBI at the break.For more baseball news, rumors and analysis, follow @cbssportsmlb on Twitter or subscribe to the RSS feed.